Figure 1 UBC-9 and SMO-1 localize to the nucleus in theC. elegans germline. (A) 3XFLAG::UBC-9 localizes to thenuclear periphery throughout the germline in the wildtype (WT) strain. Premeiotic tip (PMT) and mid/late pachytene are shown. DAPI is blue, 3XFLAG::UBC-9 is red. (B)Schematic of 3Xflag insertion in the N-terminus of theendogenous
ubc-9 locus. The tag is inserted directly afterthe start codon. (C) Endogenous SMO-1 localization inWT,
ubc-9(
tm2610), and
smo-1(
ok359) strains. Nuclear localization is present in WT, but in
ubc-9 mutants staining isdiffuse with no nuclear preference.
smo-1 mutants havelow levels of background staining with no nuclear enrichment. DAPI is blue, SMO-1 is red. (D)
smo-1::mCherry lineswith a WT sequence (GG), or a nonconjugatable pointmutation (GA), were expressed in WT and
ubc-9 backgrounds. Nuclear mCherry expression is visible in the WTsmo-1(GG) strain, but not when
ubc-9 is mutated.
smo-1(GA)strains have diffuse cytosolic staining in both WT and
ubc-9mutants. All images are midpachytene stage. (E) Graph ofsignal intensity peaks. Representative lines used for analysistaken 50% through the nucleus for SUN-1 and 3XFLAG::UBC-9 are shown.