Figure 7.
htz-1 Influences the Onset of Late Pharyngeal Gene Expression (A) Onset of R07B1.9::GFP and
myo-2::GFP after the comma stage for wild-type (black) or
htz-1(RNAi) (grey) embryos. Activation of the R07B1.9 reporter was delayed after injection of
htz-1 dsRNA (p < 0.0001, Student t-test). No RNAi: n = 18,
htz-1 RNAi: n = 12 embryos.
myo-2 activation was scored in embryos carrying either a wild-type
myo-2::GFP reporter (WT) or a
myo-2::GFP reporter with both PHA-4 binding sites mutated (Mut). Activation of the WT reporter was delayed after injection of
htz-1 dsRNA (p < 0.0001, Student t-test), whereas onset of the Mut reporter was unchanged (p = 0.2068). No RNAi: n = 15 WT, n = 33 Mut Embryos;
htz-1 RNAi: n = 9 WT, n = 21 Mut embryos. Error bars indicate the standard deviation. (B) Expression of R07B1.9::GFP at 90 and 180 min after the comma stage with
htz-1 RNAi or without (No RNAi). Pharyngeal R07B1.9::GFP is indicated by arrowheads. (C) Expression of
myo-2::GFP (WT) at 150, 200, and 250 min after the comma stage with
htz-1 RNAi or without (No RNAi). (D) Expression of
myo-2::GFP lacking PHA-4 binding sites [18] at 150, 200, 250 min after the comma stage with
htz-1 RNAi or without (No RNAi).