Fig. 7. Localization of the Arp2/3 complex. (A) Western blotting of total protein lysates from mixed- stage wild-type embryos with an anti-ARX-1 antibody (left), an anti- ARX-7 antibody (center) and a monoclonal antibody against actin (right). The band corresponding to each protein is indicated with an arrowhead. ARX-1 is at 48 kDa, ARX-7 is at 16 kDa and actin is at 45 kDa. (B) ARX-1 localization during early embryogenesis. Immunostaining with an anti-ARX- 1 antibody (a,d,g) and an anti-actin antibody (b,e,h) with merged images shown (c,f,i) for wild-type (a-c),
arx-1(RNAi) (d-f) and wsp- 1(RNAi) (g-i) embryos. (C) ARX-1 localization during ventral enclosure. Immunostaining with an anti-ARX-1 antibody (a,e,i) and an anti-actin antibody (b,f,j) with merged images shown (c,g,k) for wild-type (a-d),
arx-1(RNAi) (e-h) and
wsp-1(RNAi) (i-l) embryos. Magnified images of hypodermal cells are shown (d,h,l) for an anti- ARX-1 antibody (left), an anti-actin antibody (center) and merged images (right). (D) Expression of AJM-1 fused with GFP during ventral enclosure showing hypodermal cell shapes shortly after the time of cell arrest in embryos depleted of the Arp2/3 complex. The two extreme anterior pairs of cells are already fused. (E) ARX-7 localization during early embryogenesis. Immunofluorescent staining with an anti-ARX-7 antibody (a,d,g) and an anti-actin antibody (b,e,h) with merged images shown (c,f,i) for wild-type (a-c), arx- 7(RNAi) (d-f) and
wsp-1(RNAi) (g-i) embryos.(F) Immunofluorescence staining with an anti-ARX-7 antibody (a,e,i) and an anti-actin antibody (b,f,j) and merged images (c,g,k) of wild-type (a-d),
arx-7(RNAi) (e-h) and
wsp-1(RNAi) (i-l) embryos. Magnified images of hypodermal cells are shown (d,h,l) for the anti-ARX-7 antibody (left), the anti-actin antibody (center) and merged images (right). In C,D,F, the anterior is positioned to the left, posterior to the right and the ventral is towards the bottom. The leading edges of the migrating hypodermal cells are indicated with arrows.