Figure 3. Matefin expression is confined to the germ line from midembryogenesis to adulthood. Indirect immunofluorescence images of WT late embryo (A), WT early L2 larva (B), WT adult male (montage shown in C), WT adult hermaphrodites (D), and gonad of an adult hermaphrodite homozygous for the
gk199 allele (E), all tripled-stained with DAPI (for DNA), antibodies specific for matefin (serum 3665 for A-C or 3663 for D-E), and Ce-lamin. (A-D) The arrow indicates germ cells. Matefin did not stain mature sperm (arrowhead in C) or
gk199 homozygous gonads. Left shows DAPI staining (A and C) or Ce-lamin staining (B, D, and E). Right shows staining results for endogenous matefin. (Bars, 10 um.)