Figure 2.
sgk-1 Expression in Wild-Type and Various Mutants. (A)
sgk-1(RNAi):
sgk-1::gfp is completely downregulated in L4 larvae and adults, and strongly downregulated in younger larvae (L2 larva marked by arrows).(B-F) wild-type:
sgk-1::gfp is expressed in the cytoplasm and the nuclei of the majority of head neurons (B and C), the tail neurons PHA, PHB, and LUA (B and D), the ventral nerve cord including motor neurons (E), and the cytoplasm of the intestine (B and F).(G-I and M)
sgk-1::gfp expression in a
pdk-1(
sa680) (G-I) and
daf-2(
e1370) (M) background. SGK-1 is slightly diminished in neurons (H) and not detectable in the intestine (G, I, and M).(J-L)
daf-16(
m26):
sgk-1::gfp expression is indistinguishable from wild-type. Neither neuronal (J and K) nor intestinal (J and L) expression is affected by
daf-16(
m26).(N and O)
sgk-1::gfp expression in a
daf-7(
e1372) (N) or
daf-11(
m47) (O) background is also indistinguishable from wild-type.Same magnifications were used in (A) and (B), (G) and (J), (M)-(O); higher magnifications were used in (C)-(F), (H) and (I), and (K) and (L), always showing an L4 larva.