Figure 5. Spatial and Temporal Sites of GCY-28 Action. (A-E) Butanone chemotaxis of
gcy-28(
ky713) mutants expressing different isoforms of
gcy-28 cDNAs under various promoters. Error bars represent SEM. asterisk p < 0.05, Bonferroni t test. (A) 3.2 kb regulatory region upstream of
gcy-28.c driving
gcy-28.c cDNA. A cDNA with the
ky713 mutation is denoted c'. (B) Heat shock-inducible
hsp16-41 promoter driving
gcy-28.c cDNA. (C) Panneural H20 promoter driving
gcy-28.c and
gcy-28.d cDNAs. (D) AWC-selective
odr-3 promoter driving
gcy-28.c and
gcy-28.d cDNAs. (E) AWCON-selective
str-2 promoter driving
gcy-28.d cDNA.(F) Subcellular localization of GFP-tagged GCY-28 expressed in AWC using the
odr-3 promoter. This promoter is expressed weakly in another olfactory neuron, AWB (arrowhead).(G) Average Ca2+ responses of AWCON neurons measured by changes in G-CaMP fluorescence in wild-type and
gcy-28(
tm2411) animals. Gray shading indicates the presence of butanone. Legend indicates the dilution of butanone used as the stimulus. The left panels for each genotype show responses at the onset of the stimulus; the right panels show responses after removal of the stimulus. The shaded area around each trace represents the SEM. n = 7-11 animals for each condition.