Figure 4. SNF-11 localization: N2 (A and C) and RM2710 [
snf-11(
ok156)] (B and D) stained with alpha-SNF-11 polyclonal antibodies (goat 387; shown in green) and an alpha-UNC-17 (vesicular ACh transporter [VAChT]) monoclonal antibody (shown in red). A and B are single-channel images of alpha-SNF-11 staining, whereas C and D are merged images. Head region, anterior is to the left and ventral is down. The four RME neurons are indicated with arrows, and the processes (RMED, top and RMEV, bottom) are indicated with arrowheads. Note the punctate appearance of the processes. A second dorsal process, probably from RID, can be seen just below the RMED process. In
snf-11(
ok156) mutants, alpha-SNF-11 staining is completely absent (B and D). Mutant and wild-type images were collected and processed using identical settings. Bar, 10 um.