Figure S7, related to Figure 8.
hpo-30::gfp is expressed in PVD and FLP and negatively regulated by
ahr-1 in AVM. (A) A promoter GFP reporter for
hpo-30 (
phpo-30::GFP) is expressed in PVD and FLP neurons and in a small subset of neurons in the head and tail regions. Scale bar = 20 microns. Inset (B) shows
hpo-30::GFP expression in a FLP neuron in the head. Scale bar = 10 microns. (C) PVD lateral branches are visible with
phop-30::GFP expression. Note weaker
phpo-30::GFP expression in a subset of ventral cord motor neurons (arrowheads). Scale bar = 10 microns. The regular branching morphology of the wild-type FLP neuron (D) is disrupted (E) in an
hpo-30 mutant.
mec-3::GFP labels FLP. (F) HPO-30 is negatively regulated by AHR-1 in AVM.
hpo-30::GFP is ectopically expressed in cAVM (arrow) in
ahr-1(
ju145). (G) cAVM is colabeled with PVD::mCherry. Merged image (H) showing co-expression of
hpo-30::GFP and PVD::mCherry in cAVM. Scale bar = 10 microns.