Figure 1 The levels of BLMP-1 during development and the growth rate and lethality of
blmp-1 mutants. (A and B) The levels of
blmp-1 mRNA measured by qPCR (A) and BLMP-1 protein measured by Western blot analysis (B) are highest at L2 stage compared to other stages in wild-type C. elegans. In A, the values are average 6 SEM of three independent experiments. Em, embryos;YA, young adults. In B, actin is shown as a loading control. (C) The numbers of wild-type animals and
blmp-1 mutants in different development stages (L2, L3, L4, and dead) were counted at 48 hr from egg hatching to yield the fraction of each stage and the death rate.
blmp-1 mutants show more L2/L3 stages and dead worms compared to wildtype animals. (D) Growth rates of wild-type animals and
blmp-1 mutants. Starting at 43 hr after hatching, the numbers of adult worms were counted every hour until 100% became adults (Lee et al. 2012; Wang et al. 2015).
blmp-1 mutants showed grossly normal grow rate.