Figure 3.
ceh-23 expression is enriched in the nuclei of intestinal and neuronal cells and is increased in response to altered mitochondrial function. (A) DIC image of a rwEx19[gfp::
ceh-23+
mec-7::rfp] transgenic worm. (B) Overlay of a GFP-channel image (shown in green) and a RFP-channel image (shown in red) of a rwEx19[gfp::
ceh-23+
mec-7::rfp] transgenic worm.
mec-7::rfp transgene was used as a co-injection marker. The green fluorescence likely represents the authentic expression pattern of CEH-23. The white arrows point to the nuclei of intestinal cells. The image shown is a representative of data obtained with two independent lines. (C) Enlarged overlay (GFP and RFP) image of the head of a rwEx19[gfp::
ceh-23+
mec-7::rfp] transgenic worm showing some of the neurons expressing CEH-23. (D) Enlarged overlay (GFP and RFP) image of the posterior intestine of a rwEx19[gfp::
ceh-23+
mec-7::rfp] transgenic worm showing enriched GFP expression in the nuclei of some of the intestinal cells (white arrows). (E) mRNA expression level of
ceh-23 in wild-type worms and
clk-1(
e2519),
isp-1(
qm150);
ctb-1(
qm189), and
isp-1(
qm150) mutant worms. The mean
ceh-23 mRNA levels normalized to actin mRNA levels are shown. The mean normalized mRNA levels in wild-type worms were set as 1. Data shown were pooled from three independent experiments, p#0.05, Student t test. (F) GFP expression in
gmls-18[
ceh-23::gfp] and in
isp-1;
ctb-1;gmls- 18[
ceh-23::gfp] transgenic mutant worms at L4 stage was visualized using confocal microscopy. CEH-23::GFP expression is shown in green and fluorescent image was merged with DIC image. (G) Quantification of the GFP expression levels in
gmls-18[
ceh-23::gfp] and in
isp-1;
ctb-1;
gmls-18[
ceh-23::gfp] transgenic mutant worms. Data shown were obtained from four representative worms.