Figure 5. Expression patterns of
sma-5::gfp reporter genes. (A-C) A dorsal fluorescent image (A), a DIC image (B) and the merge of A and B (C) of an entire L4 animal transgenic for pPDW06-1a carrying a promoter for W06B3.2c. (D) A merge of dorsal fluorescent and DIC images of a young adult expressing a SMA-5/GFP fusion protein in intestine from pPDW06-9. (E) A confocal microscopic, dorsal/ventral image of a part of the body showing H-shaped excretory cell and the anterior end of intestine in an L4 worm expressing pPDW06-1a. (F) A similar confocal microscopic image, but color-coded depending on the depth, showing intestine (green), excretory cell (blue or yellow), and nuclei and faint cytoplasm of hypodermis (blue). (G,H) Lateral confocal microscopic images of an L2 worm transgenic for pPDW06-c carrying a promoter for W06B3.2a that expresses GFP in hypodermis (G) and pharynx (H).