(B,C)
egl-43 (wild type)::YFP is expressed (green) at early L2 in the pre-AC/pre-VU cells located in two different focal planes. (D,E) Either the Z1.ppp or Z4.aaa cell moves to the central position on the ventral surface of the gonad to become the AC (green, arrow), which is located (D) in a focal plane different from the two focal planes containing the three VU cells (green). Only one VU focal plane is shown in E. (F,G) The AC begins to express
cdh-3::CFP at late L2. The AC (arrow) appears yellow in F because of the co-localization of
cdh-3::CFP (red) and
egl-43::YFP (green).