Figure 4. Calcineurin in sperm and the spermatheca. (A) Immunostaining of wild-type sperm with anti-CNA-1 (top left panel) and anti-CNB-1 (bottom left panel) antibodies and the respective nuclei are shown by DAPI staining (right panels). Bar, 2 um. (B) Immunostaining of wild-type and
cnb-1 mutant spermatheca with calcineurin antibodies. Anti-CNA-1 (top left panel) and anti-CNB-1 (top middle panel) antibodies stain wild-type spermatheca. Spermatheca of
cnb-1 mutant worms do not show any anti-CNB-1 staining (top right panel). DAPI staining of spermatheca is shown in the bottom panels. Bar, 20 um. (C) Sperm and spermatheca defects. Compared with wild-type (left panel)
cnb-1 mutant worms display defects in sperm and the spermatheca.
cnb-1 sperm (middle and right panel). Arrows point to
cnb-1 sperm, which are fewer in number and show a smoother appearance compared with N2 sperm (left panel). The surrounding
cnb-1 spermatheca appears to contain debris. (D) Mating of N2 and
cnb-1 hermaphrodites with N2 males. Three N2 males were mated with one N2 or one
cnb-1 hermaphrodite for 2 days. Total progeny and outcrossed progeny are indicated by white and black bars, respectively.