(A)
nlf-1 is expressed widely in the nervous system. A fusion of the
nlf-1promoter to GFP (transgene oxEx1144) is expressed throughout the nervous system. No expression is seen in nonneuronal tissues. An L1 stage larvalanimal is shown with its head to the left. (B and C) The
nlf-1 cDNA was expressed in an
nlf-1(
ox327) mutant background using the following promoters:
rab-3 (all neurons, oxEx1146 transgene),
unc-17 (acetylcholine neurons, oxEx1149 transgene),
unc-17H (a derivative of the
unc-17 promoter that lacksthe enhancer for ventral cord expression and thus expresses in head acetylcholine neurons and occasionally a few tail neurons, oxEx1155 transgene),
unc-17b (a derivative of the
unc-17 promoter that expresses only in ventral cord acetylcholine neurons, oxEx1323 transgene),
acr-2 (ventral cordacetylcholine motor neurons, oxEx1151 transgene), and
glr-1 (interneurons including premotor command interneurons, oxEx1152 transgene).