Picture from Wenick AS et al. (2004) Dev Cell "Genomic cis-regulatory architecture and trans-acting regulators of a single ...."
Figure 7. AIY-Expressed Genes(A) Classification of AIY-expressed genes. 154 top-scoring genes from the two AIY hit lists (high-score and low-mismatch) and 100 genes ranking 10,000 were annotated and classified as shown. The 'others' class is further subdivided as shown in the Supplemental Tables.Numbers for the genome-wide annotation were extracted from Stein et al. (2003), Bargmann (1998), and Nathoo et al. (2001). Note that the under representation of putative gene-regulatory factors and metabolism/homeostasis genes in the AIY gene battery is expected from targets of terminally acting transcription factors that determine the subtype identity of a cell, but not general properties of the cell.(B) Neurotransmitter receptors that contain AIY motifs in the cis-regulatory regions. Shading in gray indicates that AIY expression was confirmed by analyzing gfp reporter fusions ([C] and Supplemental Figure S7). Note that the AIY-expressed neuropeptide receptors are not enriched for any specific receptor subtype. Percentages indicate number of genes predicted to be expressed in AIY compared to the total number of genes in the genome.(C) Examples of selected neurotransmitter receptor gfp fusions. Upper panels show complete worms, lower panels show the head region with AIY expression indicated in yellow due to an overlap of the gfp signal with the AIY-specific rfp reporter transgene otIs133. The complete set of gfp reporters is shown in Supplemental Figure S7.