Expression pattern of FOZI-1 in wild-type embryos. All images are lateral views with anterior to the left and dorsal up (unless otherwise noted). (A-B) Wild-type embryos stained with anti-FOZI-1 antibody (A) and DAPI (B). Nuclear FOZI-1 is seen in a subset of cells at the 2-fold stage of embryogenesis. (E,F) FOZI-1 staining in a wild-type L1 larva on two different focal planes. FOZI-1 was observed in the nuclei of 7-12 head neurons (arrow, E) and 5-7 cells along the ventral nerve cord (arrowheads, E,F) and in cells derived from the M lineage (red box, E,F). (G-R) Double labeling with anti-FOZI-1 antibody and hlh-8
::gfp at the 2-M (G-I), 16-M (J-L), 18-M (M-O) and 2-SM (P-R) stages in wild-type animals. Anti-FOZI-1 antibody staining is shown in panels G,J,M,P; hlh-8
::gfp is shown in panels H, K,N,Q; the corresponding merged images are shown in panels I,L,O,R. FOZI-1 staining was first detected at the 2-M stage (G-I) and persisted through the 16-M (J-L) stages. At the 18-M stage (M-O) FOZI-1 is still present in all undifferentiated CC and BWM precursors but is absent in the SMs. Arrowheads in panel M denote cells in the ventral nerve cord. (P-R) FOZI-1 is not present in the 2-SM cells and the subsequent SM lineage (data not shown). (S) Summary of FOZI-1 expression in the M lineage. The wild-type M lineage is shown with overlay of FOZI-1 expression highlighted in red.