Figure 1. Characterization of new allele deleting putative HDA-1 binding site from the
hlh-2 promoter: (A) Schematic of the endogenous GFP::
hlh-2 locus and promoter region, annotated with a previously characterized proximal regulatory element, putative HDA-1 binding site (based on published ChIP-sequencing data) and binding motif, and the genomic region deleted in the
hlh-2(
bmd231) mutant. (B) Nuclear expression of wild type (wt) GFP::
hlh-2 in the anchor cell (AC), dorsal uterine (DU) cells, and ventral uterine (VU) cells. (C-D) Nuclear expression of GFP::
hlh-2 in the AC (solid arrowhead), DU (arrows), and VU (unfilled arrowheads) cells of
hlh-2(WT) and
hlh-2(Δ-1303-702) animals. (D-E) Cytoplasmic expression of HLH-2 in the AC of
hlh-2(WT) and
hlh-2(Δ-1303-702) animals. (F-G) Expression of nhr- 67::TagRFP-T in
hlh-2(WT) and
hlh-2(Δ-1303-702) animals. (H-I) Levels of GFP::
hlh-2 following depletion of
hda-1 via RNA interference compared to empty vector control in the DU (nuclei outlined
hda-1(RNAi) micrograph). Statistical significance determined through using an unpaired two sample t-test, with Welch's correction (*p ≤ 0.05; ***p ≤ 0.001; n.s., not significant). Scale bars: 5 μm.