Figure 2. TRA-1 Has Sex-Specific Localization in the Intestine. Samples were costained with TRA-1 (left) and Mab414 (right; nuclear rim marker) antibodies.(A) In wild-type XX hermaphrodites, TRA-1 is nuclear and cytoplasmic with higher nuclear levels.(B) In wild-type XO males, TRA-1 is evenly distributed between the nucleus and cytoplasm.(C)
tra-1(
e1834null) animals do not stain for TRA-1.(D and E) Wild-type XX and XO animals expressing myc-
tra-1A transgene stained with Myc antibody. In XX animals, Myc-TRA-1A is higher in the nucleus than in the cytoplasm (D). In XO animals, Myc-TRA-1A is evenly distributed between the nucleus and cytoplasm (E).(F) Animals not carrying a myc-
tra-1A transgene do not stain for Myc-TRA-1A.(G and H) In wild-type XX L4 hermaphrodites (G) and
tra-2(lf) animals (H), TRA-1 is evenly distributed between the nucleus and cytoplasm.(I) In
tra-2(lf);
fem-1(lf) XX females, TRA-1 is nuclear and cytoplasmic with higher nuclear levels.