Figure 2. OMA-1 and OMA-2 Are Required for Nuclear Enrichment of GFP::TAF-4 in One-Cell Embryos. (A) DIC and GFP fluorescence micrographs of GFP::TAF-4-expressing embryos (first two columns), anti-TAF-4 staining of wild-type embryos (third column), and anti-GFP and anti-OMA-1 double staining of GFP::TAF-4-expressing embryos (last two columns). DC, pronuclei decondensation; PM, pronuclei meeting. Arrowhead points to pronuclei in one-cell embryos.(B) DIC and GFP fluorescence micrographs of animals expressing various GFP-fused transgenic proteins, as indicated below the DIC image. Embryos in the uterus arrange in a developmental progression from youngest (left) to oldest (right). -1, maturing oocyte.(C) GFP fluorescence in fixed embryos from transgenic animals expressing GFP::TAF-4 either without (left) or after (right)
oma-1(RNAi);
oma-2(RNAi). White arrowheads, two-cell embryos. The two-cell and two one-cell embryos not circled in non-RNAi samples are dividing.One- and 12-cell embryos are outlined in dashed and solid white lines in (B) and (C). Scale bars represent 25 μm.