Figure 4. INS-7 from URX Neurons Antagonizes INS-6 in Regulating Aversive Olfactory Learning (A) Schematic for C. elegans head neurons. (B) The expression reporter yxIs13[Pins-7::gfp] is expressed in head neurons. (C-E) yxIs13[Pins-7::gfp] is expressed in ASI, ASK (C), ADF (D), and URX (E) neurons. Red fluorescence was generated from DiI-staining of several sensory neurons (C) or expression of neuron-specific reporters of mcherry (D,E). (F and G)
ins-7 expression in ADF or ASI (Psrh-142::
ins-7 or Pstr-3::
ins-7, respectively, in F) or in RIM (Pgcy-13::
ins-7 in G) did not alter the learning ability of
ins-6(
tm2416);
ins-7(
tm2001) double mutants. The learning ability of transgenic animals was compared with that of nontransgenic siblings using the paired Student's t test. n.s., not significant; n R 5 assays; mean +/- SEM. (H-J)
ins-7 expression in URX using the
gcy-36 promoter at 25 ng/ml in (H) or 2 ng/ml in (I) or using the
flp-8 promoter at 25 ng/ml in (J) reverted the normal learning of
ins-6(
tm2416);
ins-7(
tm2001) double mutants back to the defective learning of
ins-6(
tm2416) single mutants. The learning ability of transgenic animals was compared with that of nontransgenic siblings using the paired Student's t test. **p < 0.01; n R 5 assays; mean +/- SEM.