(B) Epifluorescence smFISH images depict localization of
cki-1 transcripts (grayscale, top and red in overlay, bottom), nuclei (grayscale, second row and blue in overlay, bottom), and an AC-specific reporter (
lin-3>NLS-GFP, grayscale, third row and green in overlay, bottom). The AC in the
nhr-67(RNAi)-depleted animals is undergoing mitosis and thus lacks DAPI staining and an intact nucleus. Thus, the NLS-GFP is present throughout the cell. The white lines indicate the position of the AC and the dashed yellow lines indicate the position of the AC nuclei.(C) Quantification of
cki-1 mRNA transcripts per AC by smFISH (n > 10 animals examined for each, *p < 0.002, by a Student's t test, and error bars represent SEM).