Picture from Reilly DK et al. (2019) microPublication Biology "Evolution of hermaphroditism decreases efficacy of Ascaroside#8-mediated ...."
Figure 1 Caenorhabditis species males respond differentially to ascr8. (A) Dwell times of Caenorhabditismales in vehicle control (-) and ascr8 (+), respectively. Hermaphroditic species were tested alongside theirgonochoristic sister-species (denoted by dotted horizontal brackets: C. elegans vs. C. inopinata, C. briggsae vs. C.nigoni, C. tropicalis vs. C. wallacei). The Japonica Group species, C. japonica and C. afra, (left of vertical dottedline) were also tested for their attractive response. Species are displayed according to the most recent phylogeneticanalysis of the Caenorhabditis genus (Stevens et al., 2019). No species were as attracted to ascr8 as C. elegans,although C. briggsae, C. nigoni, and C. wallacei did spend significantly more time in ascr8 compared to the vehicle. Error bars denote SEM. n 12. Paired t-tests or Wilcoxon test (dependent on Normality test) of ascr8 vs.vehicle control. (B) The Log2 of Transformed fold-change of ascr8 vs vehicle dwell times. See Description for transformation calculations. Among the sister-species pairs, gonochoristic species exhibited a significant increase inascaroside dwell time. Student's t-test. Brackets linking species denote sister-species pairs, with androdioeciousspecies on the left, gonochoristic on the right. * p < 0.05, **** p < 0.0001.