Figure 1. Cytological and phenotypic characterization of
cosa-1:mCherry
(wsh7):(A) Fluorescence images showing the localization of the COSA-1::mCherry foci in the different zones of the germline. White rings indicate examples of 6 COSA-1::mCherry foci per nuclei in the late pachytene (LP) of adult worms, blue arrows define germline distal to proximal direction (MZ - mitotic zone, TZ - transition zone, EP - early pachytene, MP - mid pachytene, LP - late pachytene, DP -diplotene). (B) Colocalization of GFP::COSA-1 and COSA-1::mCherry. (C - E) Analysis of brood size (C), progeny viability (D) and male frequency (E) in the
cosa-1::mCherry
(wsh7) transgenic strain compared with the wild type (ns - not significant; Independent samples t-test, n= 26).