Figure 4.
syg-2 Is Expressed in the Primary Vulval Epithelial Cells during L3 and L4 StagesSYG-2::GFP is a biologically active GFP fusion at the C terminus of a 16 kb genomic
syg-2 clone. Images are taken from animals carrying the extrachromosomal array kyEx684 (A, B, E, F, I, J, M, and N) or animals of equivalent age expressing SYG-1::GFP (kyIs288) (C, D, G, H, K, and L).(A and B) Fluorescence and DIC images of a mid-L3 stage kyEx684 animal. Arrows point to the four primary vulval epithelial cells.(C and D) SYG-1 GFP and DIC image of an animal at the same stage as (A) and (B).(E and F) Fluorescence and DIC images of a mid-L4 stage kyEx684 animal. Arrows point to the four primary vulval epithelial cells on the left side of the animal. Expression of GFP in the primary vulval epithelial cells is higher than in (A). Low expression can be seen in body wall muscles.(G and H). SYG-1 GFP and DIC image of an animal at the same stage as (E) and (F).(I and J) Fluorescence and DIC images of a young adult kyEx684 animal. Note absence of expression in the vulval cells, and faint fluorescence in vulval muscles.(K and L). SYG-1 GFP and DIC image of an animal at the same stage as (I) and (J). Arrows in (K) point to diffusely localized SYG-1 on HSNL axon outside of synaptic regions.(M and N) Fluorescence and DIC images of a mid-L4 stage
lin-11(
n566); kyEx684 animal. Note absence of expression in the primary vulval epithelial cells. Low expression of GFP can be seen in body wall muscles, similar to that of the wild-type animal in (E).