Figure 1. Intracellular localization of olfactory Ga-subunits. In adult C. elegans (A), the olfactory Ga-subunits locate to specific compartments of the amphid neurons. (B) The location and structures (dd, dendrite; cb, cell body; ax, axon) of the amphid neurons. GPA-2 (C) localizes to cilia (arrow) and cell bodies (arrowheads) of amphid neurons. GPA-13 (D) localizes to amphid cilia (arrow). GPA-5 (red) can be observed in the cilia (arrow), axons (open arrow), and cell bodies (arrowhead) of the AWA cells (E) and is localized nearby
snb-1::GFP (green) in the axons (F, open arrows). GPA-6, in gpa-6XS animals, was not detected in the cilia, but in the dendrites (arrows), cell bodies (arrowhead), and axons (open arrow) of four pairs of amphid cells (G). GPA-3 localizes to the cilia of many amphid cells (H). GPA-3 staining in the rod-like cilia is clearly visible (arrow), surrounded by the more fuzzy wing-shaped AWA and AWC cilia staining (arrowheads). Bars: C, D, E, G, and H, 20 um; F, 5 um. (A-H)
x400.