Figure 4. Localisation of
mab-9. CCD images ofmab-9::GFP fluorescence. Posterior is to the right; dorsal is up. (A) (Top left) Hermaphrodite L1 larva, lateral view. (Top right) Tracing with the position of the rectum marked (arrowhead) and the relative positions of B and F nuclei shown. The white arrowhead (top left) indicates one of a pair of neuronal nuclei expressing
mab-9::GFP, slightly out of the focal plane. The two posterior
hyp7 nuclei are marked with asterisks (*). (Bottom left) Male L1 larva. Note the larger size of the B nucleus. (B) L2 male. B has divided into the larger B.a and the smaller B.p, and GFP expression is seen in both daughters. B does not divide in hermaphrodites. (C) Ventral view, hermaphrodite L3. The pair of neuronal nuclei is now clearly visible in the focal plane (white arrowheads). The relative positions of the B and F nuclei are shown in the tracing at right. (D) Late L4 male, ventral view. B lineage nuclei of cells associated with the spicule expressing
mab-9::GFP can be seen in this focal plane. A Nomarski photomicrograph of this image is shown at right, with the spicules marked by a white arrowhead. (E) A 1.5-fold embryo expressing
mab-9::GFP in three posterior cells around the presumptive rectum. Nomarski image shown atright. (F) Three-fold embryo expressingmab-9::GFP. Bright fluorescence is observed in the B and F rectal cells (white arrowhead). Expression is also observed transiently in ventral cord nuclei. Nomarski image shown at right (embryo is moving by this stage).