Figure 7. Expression pattern of ace-1
::GFP in muscle cells. In these experiments, animals were injected with pCe 4079 (Figure 6). Transformants were identified by the roller phenotype due to the co-injected vector pRF4. (a) General view of an adult transformant ( 100). Head at left, ventral side down. Body wall (mc) and pharyngeal muscle cells pm5
are fluorescent. There are three elongated pm5
(binucleated) cells that cover the isthmus region of the pharynx from the metacorpus to the terminal bulb. The bright fluorescence dorsal and posterior to the pharynx bulb comes from the head mesodermal cell (hm), a mesodermal cell with neuronal like processes. In the tail, anal muscles (am) are fluorescent (see (c)). Note the helical twisting of muscle quadrants characteristic of rol-6
phenotype. (b) Detail of a bundle of mononucleated body wall muscle cells ( 400). Inset: higher magnification of a muscle cell showing characteristic striation ( 630). (c) and (d) High magnification ( 400) of the anal sphincter muscle (arrow in (c)) and of the four vm1
muscle cells of the vulva (arrow in (d)). A similar pattern of expression was obtained with shorter fragments of 5' region of ace-1
in C. elegans (see PCe2398a and pCe2398b of Figure 6), and C. briggsae (see pCb2373 in Materials and Methods).