Figure 1. : A. Top: Cartoon depicting the protein structure of ZHP-3 with phosphorylated residues indicated. RFM indicates the ring finger motif. Bottom: Gel stained with Coomassie blue and autoradiograph of in vitro kinase assays with N- and C-terminal regions of ZHP-3 and human Chk1. B. Mid pachytene nuclei stained with ZHP-3 (green) and SYP-1 (magenta) antibodies in wildtype germlinesS308A S366A S308A, S366Aand
zhp-3 (
jf61) null,
zhp-3 ,
zhp-3 , and
zhp-3 mutant germlines. C. Late pachytene nuclei stained with ZHP-3S308A S366A S308A, S366A (green) and SYP-1 (magenta) antibodies in wildtype germlines and
zhp-3 (
jf61) null,
zhp-3 ,
zhp-3 , and
zhp-3 mutant germlines. D. Quantification of average number of DAPI bodies in wildtype oocytes and
zhp-3 (
jf61) null,
zhp-3 , zhp-S366A S308A, S366A3 , and
zhp-3 oocytes. E. Bivalents stained with DAPI and antibodies against HTP-3 (magenta) and HTP-1 (green) inS308A S366A S308A, S366Awildtype oocytes and
zhp-3 (
jf61) null,
zhp-3 ,
zhp-3 , and
zhp-3 mutant oocytes. F. Quantification of embryonicS308A S366A S308A, S366Aviability and total number of male progeny in wildtype,
zhp-3 (
jf61) null,
zhp-3 ,
zhp-3 , and
zhp-3 strains. Allscale bars indicate 5 microns. Significance was assessed using two-sided Wilcoxon-Mann-Whitney tests.