Figure 7. Immunofluorescent localization of SPE-39 during spermatogenesis in males. All worms were in a
dpy-11 genetic background and the genotype with respect to
spe-39 is indicated on the six leftmost panels. From left to right, each row has a series of four corresponding images: a DIC image, DNA visualized by DAPI staining (blue), staining with the MO-specific monoclonal antibody 1CB4 (green), and staining with the anti- SPE-39 antibody N8 (red). (A-D) Wild-type spermatocyte in the process of spermatid budding. The arrows point to budding spermatids (s) and the anucleate residual body (rb). (E-L)
spe-39 mutant spermatocytes. Occasionally, unsuccessful attempts to bud spermatids are observed (arrows in I). (M-P) A wild-type spermatid and (Q-X)
spe-39 mutant spermatids. Nuclei are located off center in
spe-39 spermatids (arrows in Q and U). Bars, 5 um.