Figure 3 (A) Schematic of
sma-1 gene, indicating the newly isolated nonsense mutation,
iv38, and the structure of the
sma-1::GFP reporter; a and b designations indicate start positions of each of two
sma-1 isoforms. (B) Expression of
sma-1::GFP in the posterior pharyngeal bulb. Expression is visible in the myoepithelial cells but is absent from pharyngeal glands (arrowheads). (C-E) Nuclear
sma-1::GFP::HIS2B is absent from gland cells; dorsal view of posterior pharyngeal bulb (outlined). (C) sma- 1::GFP::HIS2B is evident in nuclei of pharyngeal muscles and marginal cells; arrowheads indicate weak GFP signal from out-of-plane
pm5 pharyngeal muscle nuclei. (D)
phat-1::wCherry in
g1A glands, with dotted circles showing position of
pm5 nuclear signal; no nuclear GFP is evident within the gland cells. (E) Merge of images from C and D, with
pm5 nuclei outlined. (F and G) Gland morphology in
sma-1(
e30) mutants resembles that seen in
sma-1(
iv38) mutants.