Figure 3.
mir-84::gfp and
gfp60 Are Expressed Reciprocally in the Pn.p Cells. (A, C, E, and G) In L3,
mir-84::gfp is expressed in all Pn.p cells except P6.p during the time of vulval fate determination ([O], bracket) and is upregulated in P6.pxx cells thereafter (mL3, lL3, and vlL3 are mid, late, and very late L3, respectively).(I, K, and M)
gfp60, a fusion of gfp to the
let-60 3'UTR, driven by the
lin-31 promoter, is expressed in all Pn.p cells at late L2 and early L3 (I and J), is then restricted to P6.p (K and L) during vulval fate determination, and thereafter is expressed strongly in P6.px cells and weakly in P5.px and P7.px cells (M and N). Images on right are Nomarski images of animals on left.(O)
gfp60 and
mir-84::gfp expression schematic from L2 Pn.p to mid L3 Pn.px.(P-R), Quantification of expression data shown in (A-N).
gfp54 is a fusion of gfp to the
unc-54 3'UTR, driven by the
lin-31 promoter.Error bars represent standard deviations. See Experimental Procedures for details.