Fig 5. SLO-1 and SLO-2 BK potassium channels are localized in the vicinity of UNC-2 voltage-gated calcium channels in AWC axons. (A-C) Images of wild-type L1 animals expressing single copy insertion transgenes
odr-3p::
slo-1::TagRFP and
odr-3p::GFP::
unc-2 (A),
odr-3p::
slo-2::TagRFP and
odr-3p::GFP::
unc-2 (B), as well as
odr-3p::
slo-2::TagRFP and
odr-3p::
slo-1::GFP (C) in AWC neurons. SLO-1::TagRFP(A), SLO-1::GFP (C), SLO-2::TagRFP (B, C), and GFP::UNC-2 (A, B) were localized in AWC cell bodies (arrows) and in a punctate pattern along AWC axons (arrowheads). In AWC axons, SLO-1::TagRFP was localized next to GFP::UNC-2 (A); SLO-2::TagRFP was adjacent to GFP::UNC-2 (B); and SLO-2::TagRFP was localized near SLO-1::GFP (C). Insets show higher magnification of the outlined areas that exemplifylocalization of two translational reporters in close proximity. Scale bar, 5 um. Anterior is at left and ventral is atbottom. (D) Quantification of mean correlation coefficient between SLO-1 and UNC-2, SLO-2 and UNC-2, as well as SLO-1 and SLO-2 using three algorithms of the Coloc 2 plugin in Fiji: Pearson's correlation coefficient, Spearman's rank correlation coefficient, and Li's ICQ. For each colocalization class, images of three animals were used for quantification. Positive values of each coefficient indicate positive correlation, values close tozero indicate no correlation, and negative values indicate anti-correlation. Pearson's correlation coefficient ranges from -1 to +1; Spearman's rank correlation coefficient ranges from -1 to +1; Li's ICQ value ranges
from-0.5 to +0.5. A schematic diagram of the AWC cell body, axon, dendrite, and cilia that represents the approximate region of images in A-C is shown in S2D Fig.