Figure S1. Related to Figures 1 and 2.(A) Phylogenetic tree of DEAD box and related RNA helicases in C. elegans.(B) Protein models of ZC317.1, MUT-14, and SMUT-1, including Interpro predicted motifs.(C) Diagram of ZC317.1 based on Wormbase prediction and ModEncode RNA-Seq data.(D) Assay for RNAi defects in ZC317.1 mutants.(E) Protein blot assays for GFP from the
ubl-1::GFP control transgene and the
ubl-1::GFP-siR-1-sensor transgene in wild type and mutantanimals. Actin protein is shown as loading control. The bar graph displays the mean +/- SD normalized GFP protein levels from threebiological replicates.(F) GFP fluorescence from
ubl-1::GFP-siR-1-sensor transgene in wild type and
mut-14(
pk738)
smut-1, ZC317.1, and ZC317.1
mut14(pk738)
smut-1 mutants.(G) Taqman quantitative RT-PCR assay of siRNA levels in wild type and mutant animals. Error bars display standard deviation from themean for three biological replicates. P values are from comparisons to wild type. The CSR-1 class siRNA is derived from
hcp-1 and theWAGO class siRNA is derived from B0250.8 (Table S5).(H) mCherry expression from
smut-1 and
mut-14 promoter fusions in adult animals. The gonads are outlined in magenta. Arrows point todeveloping embryos.