Figure 6. SET-25 Localizes to Perinuclear Foci in an H3K9 Methylation-Dependent Manner (A) SET-25 and MET-2 were tagged N terminally with mCherry (mCh) and expressed in C. elegans embryos under control of the ubiquitously active
his-72 promoter. Scale bar, 5 mm. (B and C) Representative nuclei of embryos expressing mCh-SET-25 as described in (A) under control and
sams-3 RNAi conditions. mCh-SET-25 forms perinuclear foci in the absence (B) and presence (C) of the gwIs4 array. Strong SET-25 foci in C colocalize with the GFP-LacI signal that marks the gwIs4 transgene. Peripheral SET-25 foci and array-associated SET-25 are dispersed upon
sams-3 RNAi. (D) Quantification of the radial distribution of mCh-SET-25 foci as shown in B (top) by three-zone scoring (Figure 2E). SET-25 foci are significantly enriched at the nuclear envelope over a random distribution (dotted line, p < 1015, Fisher's exact test). (E) Localization of mCh-SET-25-C645A mutant in wild-type and
met-2(
n4256)
set-25(
n5021) embryos. (F) As in (C, bottom), but for mCh-SET-25DSET and in wild-type,
set-25(
n5021) and
met-2(
n4256)
set-25(
n5021) embryo. (B, C, E and F). Scale bar, 2 um.