Figure 2. Rescue and expression pattern of
ser-1. A, Rescue of the resistance to 5-HT- induced egg laying in
ser-1 mutant animals in liquid. Three transgenic
ser-1 strains containing the
ser-1 open reading frame and a 7 kB upstream promoter (
ser-1;egEx18[
ser-1(+)],
ser-1; egEx19[
ser-1(+)],
ser-1;egEx20[
ser-1(+)]) showed a restored 5-HT-induced egg-laying re- sponse. The control strain (
ser-1;egEx23[Pofm-1::gfp]) carrying an array containing only the coinjection marker,
ofm-1::gfp, showed no rescue of the
ser-1 mutant egg-laying behavior nor did the strain
ser-1;egEx33[
ser-1(+);Pofm-1::gfp] containing a 3.4 kB upstream promoter and
ser-1 open-reading frame. Overexpression of
ser-1 in a wild-type background (egEx18[ser- 1(+)]) behaved like wild-type for 5-HT-induced egg laying. Selective expression of
ser-1 in muscles (
ser-1;egEx26[Pmyo-3::
ser-1(+)]) restored the 5-HT-induced egg-laying response. Single wild-type animals were placed into liquid M9 buffer containing 12.5 mM 5-HT for 90 min, and the number of eggs laid was counted. n = 24 for each group. Error bars indicate SEM. The asterisk indicates a statistical difference from
ser-1 mutant animals ( p < 0.001), and the number sign indicates a statistical difference from wild-type animals (Mann-Whitney U test). B, C, Expression pattern of Pser-1::gfp in L1 hermaphrodite larvae. Pser-1::gfp(egEx21) contains an 8 kB upstream
ser-1 promoter and is expressed in pharyngeal muscle, neurons in the head, ventral cord, and tail of L1 larvae. D, Expression of Pser-1::gfp(egEx21) in young adults. Pser-1::gfp is expressed in the vulval muscles in young adult hermaphrodites.