Figure 9. Hypodermal expression of
lin-29::lacZ reporter fusions. Strains transgenic for
lin-29A::lacZ and
lin-29B::lacZ were assayed for -galactosidaseaccumulation by indirect immunofluorescence, as described in Materials and Methods. Both constructs program hypodermal expression beginning during the L2 stage. (A)
lin-29 gene structure. The horizontal line depicts the
lin-29 locus with respect to EcoRI restriction sites (RI). The
lin-29A and
lin-29B transcripts are shown above this line. The boxes represent exons. Filled boxes are protein coding regions.
lin-29A exons are numbered 1-11. Exon 1 of
lin-29A is trans-spliced to SL1. The
lin-29B-specific exon is referred to as exon 1b. The remaining exons are shared with
lin-29A and are referred to as exons 5b-11b. cDNA analysis has revealed that
lin-29B can also be trans-spliced to SL1 at the start of exon 5b (Rougvie and Ambros, 1995).
lin-29 gene fragments used in lacZ reporter gene constructs are shown below the restriction map (see Materials and Methods). (B) A L3 larva transgenic for
lin-29A::lacZ and stained for beta-galactosidase and MH27.