Figure 1. FP fusions to predicted ER membrane proteins can be localized to the ER in C. elegans. (A) YFP-TRAM (YTRAM) was expressed in body wall muscle. A single confocal plane is shown, and the NE and surrounding reticulum are visible. (B) In head muscle markers for different cellular membranes are easily distinguishable. YFP-SP12 (YSPR), YFP-mannosidase transmembrane and stalk (Ymans), YFP-emerin (Yemr), and YGPI were expressed under the control of the
glr-1 promoter and imaged in head muscles. In the Ymans and YSP12 panels the nucleus is at the right, and the anterior contractile region of the cell is on the left. Scale bars, 5 um.