Figure 1. Localization of HIM-19:3xFLAG in the C elegans germline:A) Co-immunodetection of HIM-19::3xFLAG and nuclear envelope protein SUN-1 phosphorylated on serine 24 (SUN-1 S24-Pi), a marker of nuclei with active CHK-2, in a whole-mount gonad from phenotypically wild-type worm expressing 3xFLAG-tagged HIM-19 from the endogenous
him-19 locus. White arrowheads indicate "outlier" nuclei with persistent CHK-2 activity; scale bar represents 20µm. B) Zoomed-in images of nuclei from the regions indicated by white boxes in A. Orange arrowheads indicate nuclei in which bright HIM-19::3xFLAG signals coincide with sites of SUN-1 S24-Pi enrichment; scale bars represent 2µm. Images in A and B are maximum intensity projections through the top layer of nuclei of the gonad, except for the mitotic zone image in B, in which a single equatorial slice is depicted to highlight the localization of HIM-19::3xFLAG at the nuclear periphery.