Figure 4. Expression of other DYRK1A/minibrain family members. (A-F) MBK-2::GFP is excluded from the nucleus and is expressed in the nervous system. Expression constructs are shown in Figure 1. (A) In L1 larvae, the 5' noncoding sequence of
mbk-2L drives gfp expression widely in the nervous system, including head neurons (arrowhead) and the ventral nerve cord (arrow). (B) In adult animals, gfp continues to be expressed in the nervous system (arrow) and is also expressed in body wall muscle (arrowhead). (C) A translational fusion between
mbk-2S and gfp is expressed at the periphery of the pharynx (arrow). (D) Induced expression of a translational fusion protein between
mbk-2L and gfp also localizes to the periphery of the pharynx (arrow). In addition, the protein is clearly excluded from the nuclei of neurons in the head (inset, arrowheads); nuclear exclusion was further corroborated by DAPI staining of an embryo (F) that expresses
mbk-2::gfp (E). (G-K) HPK-1::GFP expression. The expression construct is shown in Figure 1. To monitor GFP expression in living embryos, two-photon laser scanning microscopy was used on mounted embryos. (G) A translational fusion protein between
hpk-1 and gfp localizes to nuclei and is widely expressed in cleavage stage embryos. (H) After 4 min, the number and location of HPK-1-GFP puncta within the embryo has dramatically changed. (J) The boxed region is shown at 1-min intervals. The puncta within the highlighted nucleus rapidly rearrange (arrowheads). (K) A fluorescence micrograph of HPK-1-GFP at postembryonic stages. In adult animals, the number, intensity, and movement of HPK-1-GFP puncta (arrowhead) are greatly reduced.