Figure 1. Retraction Angle distinguishes Ore from non-Ore male tail tip phenotypes:Newly hatched CB4088
him-5(
e1490) males were fed bacteria carrying either the L4440 control plasmid or a
lin-41 RNAi- inducing plasmid and imaged as early adults. (A, B) Arrowhead indicates location of cloacal opening. Vertical yellow line is the cloacal axis, perpendicular to the anterior-posterior axis of the worm and originating at the cloacal opening. (A', B') Arrow indicates the posterior position of the male tail tip. Lines connect the male tail tip to the cloacal axis at the cloacal opening. The curve represents the Retraction Angle (RA). (C) The mean Retraction Angle among
lin-41 RNAi males is significantly lower than that of the L4440 control. Each dot represents an individual male worm. Graph shows mean and standard deviation. (Student's t-test, **** p<0.0001). (D) Penetrance and severity of retraction phenotypes defined with respect to the mean RA of L4440 RNAi males where non-Ore male tails had an RA <1 standard deviation from the mean of the L4440 RNAi population, mild Ore had an RA between 1-2 standard deviations from the L4440 mean, and severe Ore had an RA >2 standard deviations from the L4440 mean (Fisher exact test, Ore and non-Ore, **** p<0.0001). (E) Penetrance of the Ore phenotype, defined as an RA less than the lowest RA in the L4440 population (47o) (Fisher exact test, Ore and non-Ore, **** p<0.0001).