Fig 2. Akirin regulates multiple nlp genes in the epidermis. A. Confocal images of IG1485 transgenic worms expressing an
akir-1p::gfpreporter gene showing epidermal and neuronal expression of GFP. The lateral epithelial seam cells are indicated by the arrowheads. Much ofthe fluorescence in the head and tail comes from neurons, seen more clearly in the right panel. Scale bar 50 um. B. Ratio of green fluorescence(GFP) to size (TOF) in
rde-1(
ne219);
wrt-2p::RDE-1 worms that are largely resistant to RNAi except in the epidermis, carrying the array frIs7,treated with RNAi against the indicated genes and infected by D. coniospora. The black bar represents the mean value for (from left to right),n = 135, 49, 155, 102, 130, 94; p<0.0001, Dunn's test. C. Quantitative RT-PCR analysis of the expression of genes in the
nlp-29 cluster inrde-1
(ne219);
wrt-2p::RDE-1 worms treated with RNAi against the indicated genes and infected by D. coniospora; results are presented relativeto those of uninfected worms. Data (with average and SD) are from three independent experiments (S2B Fig). , p < 0.001; , p < 0.01;1-tailed ratio paired t-test