Figure 1. Synaptic Localization of RAB-27. (A)
rab-27 is expressed in the nervous system. Animals carrying a transgene expressing GFP under the
rab-27 promoter. Expression is seen in the neuronal ganglion (arrow) and intestinal cells (arrow head). Bar, 20 um. (B) Colocalization of RAB-3 and RAB-27 in synapse-rich regions. Wild-type animals costained with RAB-3 (green) and RAB-27 (red) antisera. Immunoreactivity is most apparent at synapse-rich regions of the nerve ring. Bar, 10 um. (C) Colocalization of RAB-3 and RAB-27 at the synapse. Wild-type animals costained with RAB-3 (green) and RAB-27 (red) antisera. RAB-3 and RAB-27 seem to colocalize within the synapse-rich ventral cord (VC), although their colocalization is not complete. Bar, 10 um. (D) RAB-27 synaptic localization is independent of RAB-3.
rab-3(
js49) mutant animals were stained for RAB-27. Immunoreactivity remained abundant in the synapse-rich regions of the dorsal cord (DC) and VC. Bar, 10 um. (E) RAB-27 localization is UNC-104 dependent. Wild-type and
unc-104(
e1265) mutant animals stained with RAB-27 antisera. RAB-27 staining is found in cell bodies anterior and posterior to the synaptic-rich nerve ring in
unc-104 mutant animals. Bar, 10 um.