Figure 2. Expression Patterns and Fibrous Organelle Phenotypes of Selected evab Genes (A) Conserved domains of EEL-1 and CRT-1. Horizontal black bars indicate extent of deletion alleles ok1575
; arrow indicates position of GFP insertion. The following abbreviations are used: DUF, domain of unknown function; UBA, ubiquitin-associated domain; HECT, homologousto the E6-AP carboxyl terminus domain. (B) A transcriptional eel-1
::gfp construct is expressed at low levels in most cells. (C-J) Immunostaining with NE8/4C6 (muscle; red) and VAB-10A (green) antibodies of vab-10A
) (C and H), crt-1
) (D), eel-1
) (E), vab-10A
) (F and I), and vab-10A
) embryos (G and J) at 1.7-fold (H-J) or 3-fold (C-G) stages. Antibodies from boxed areas in (Fa) and (Ga) are enlarged in (Fb) and (Fc) and (Gb) and (Gc), respectively. Arrows in (Fb) and (Gb) indicate areas with detached muscles but normal VAB10A; arrowheads in (Fb) and (Gb) indicate areas with abnormal VAB-10A; dotted lines in (Fc) and (Gc) indicate expected muscle positions; arrowheads in (I) and (J) indicate areas with detached muscles. Scale bar in (C) represents 10 um.