Figure 4. CeY RNA is transcribed at higher levels in the adult germline and in embryos. Total RNA was prepared from staged worms and 5 ug of total RNA was resolved by formalde- hyde-agarose gel electrophoresis, transferred, and probed with (A) a
rop-1 cDNA, (B) the
yrn-1 gene, or (C) a portion of the gene encoding actin. The levels of
rop-1 mRNA and CeY RNA are both high in the embryos, but decrease at the subsequent larval stages. However, the levels of CeY RNA increase a little at the L4 stage and a lot at the adult stage. Lane 1, wild-type embryos; lane 2, wild-type L1 larvae; lane 3, wild-type L2 larvae; lane 4, wild-type L3 larvae; lane 5, wild-type L4 larvae; lane 6, wild-type nongravid adults; lane 7,
glp-1(
q231) mutant adults. (D) The intensity of each band obtained in (B) was deter- mined by scanning the X-ray film with a LKB ultroscan XL enhanced laser densitometer (Pharmacia, Piscataway, NJ). Each individual band in (B) was corrected with the densitomet- ric value obtained by scanning the band in the corresponding lane obtained in (C) with an actin probe. The corrected data were plotted on a graphical chart.