Figure 3
cdk-8 acts upstream and downstream of
let-23/EGFR. (A) qPCR analysis of
lin-3 mRNA levels in
cdk-8 single mutants and mutants with synMuv genes, relative to wild-type worms. lin-15AB mutant is shown as a positive control (hatched bar). Error bars represent standard error of the mean (SEM, n = 3 independent trials). No statistically significance differences, Wilcoxon signed rank test by the Pratt method. (B) hdEx508[
cdk-8P::GFP] expression in the anchor cell (arrow) during early vulval induction. Top: VPCs divided once (Pn.px). Middle: VPCs divided twice (Pn.pxx). Bottom: Invagination of Pn.pxx epithelium. The fluorescent signal visible near VPCs localizes to neuron cell bodies. Bar: 10 um. (C) Average fluorescence intensity of syIs107[lin-3ACEL::GFP] in anchor cell of wild-type worms and
cdk-8 mutants. *P , 0.05 vs. N2, t-test. A.U., arbitrary units. (D) L4 Vul penetrance incdk-8;
let-23/EGFR and
cdk-8;
mpk-1/ERK mutants (n $ 8). ****P , 0.0001, Fisher's exact test. See Table 1 for raw data. (E) Tissue specificity of Muvphenotype in
cdk-8;
lin-15A mutant adults expressing wild-type
cdk-8 driven by its own promoter
cdk-8(+), the Pn.p promoter
lin-31P, or the hypodermal promoter
dpy-7P, compared to nontransgenic siblings (Sib) in each strain (n $ 76). ****P , 0.0001 vs. nontransgenic sibling, Fisher's exact test. See Table S2 for raw data.