Figure 8. Pattern of
gly-2promoter-directed GFP expression during nematode development. Except for panels F and G, images are presented as pairs showing the epifluorescence from the cytosolic GFP reporter in the upper section and differential interference contrast images from the same field and focal plane in the lower section. The emerald-green light is emitted by GFP, the yellow-green signal is gut autofluorescence. Scale bar in all cases is 20 μm.A, comma stage embryo. B, 1.5-fold stage embryo.C, 3-fold stage embryo. D, lateral view of an early larval stage 4 hermaphrodite peri-vulval region. DNC, dorsal nerve cord; VNC, ventral nerve cord; vul, vulval cells vul E and/or vul F. E, ventral view of late larval stage 4 hermaphrodite peri-vulval region. SV, spermathecal valve; v/u, vulval and/or uterine structures.F, as E, but from a nuclear-localized reporter.G, detail in lateral view of the spermathecal valve staining by the nuclear-localized reporter expressed in an adult hermaphrodite.H, lateral view of an adult hermaphrodite mid-body showing continued expression in the spermathecal valve (SV) and vulval and/or uterine structures (v/u). I and J, lateral views in two different focal planes from adult hermaphrodites showing vulval/uterine staining (v/u).K, lateral view of the pharynx from an adult hermaphrodite showing GFP fluorescence from neurons (N) in the nerve ring and ventral nerve chord (VNC) and the staining of the pharyngeal-intestinal valve (PIV).