Figure 5. SMF-1 is expressed in DA neurons. Primary C. elegans cultures expressing GFP in the DA neurons were generated with WT (A-D) or
smf-1 mutants (E-H), and fixation was performed as described under Experimental Procedures. Primary cultures were incubated with SMF-1 primary antibodies followed by incubation with Texas Red-conjugated goat anti-rabbit secondary antibodies. Differential interference contrast images of A and E of WT and
smf-1 mutant cultures, respectively, are shown. DA neurons from WT and
smf-1 mutants expressing GFP driven by the
dat-1 promoter B and F, respectively. SMF-1 colocalized with DA neurons in WT animals (C), but not in
smf-1 mutants (G). D, overlay of B and C; H overlay of F and G. Images were observed under a Zeiss confocal microscope (Zeiss LSM 510). Scale bar represents 45 μm.