Figure 1. MPS-4 is expressed in the pharynx.A, MPS-4 protein sequence and alignment with KCNE1 (64% homology). Red, green, and blue colors indicate, respectively, identical, strongly, and weakly similar residues. Alignment was done by ClustalW (available at bioweb.pasteur.fr). B, phase-contrast light transmission image of a pharynx of a exp::gfp transgenic nematode used in B-E. C, immunofluorescence image of the pharynx in A stained with anti-MPS-4 primary antibody and Cy3-conjugated goat anti-rabbit secondary antibody. D, fluorescence image microscopy of the same pharynx in A. The green fluorescent signal represents
exp-2 expression. E, merge of the images in A and B. F, immunofluorescence image of the pharynx of a
mps-4 KO nematode stained with anti-MPS-4 antibody. Images were taken with an Olympus BX61 microscope equipped with a digital camera and analyzed with dedicated software.