Figure 3. Recruitment of DPY-21 to X Chromosomes Is Cell-Cycle Dependent and Coincides with H4K20me1 Enrichment(A) Nuclei from wild-type and
dpy-21(
y607 JmjC) XX embryos of different ages stained with DAPI and H4K20me1, SDC-3, and DPY-21 antibodies. DPY-21localizes to X later in development than other DCC subunits, coincident with H4K20me1 enrichment on X. DPY-21 localized to X with similar timing in
dpy21(JmjC) and wild-type embryos. Yellow arrows, foci of SDC-3, DPY-21, or H4K20me1 on X. Red arrows, diffuse nuclear localization of H4K20me1 or DPY-21.Scale bar, 2 um.(B) Individual nuclei of a wild-type 376-cell embryo reveal cell-cycle-dependent association of DPY-21 on X during interphase, but not mitosis, unlike other DCCsubunits. Scale bar, 1 um.See Figures S3 and S4.