Figure 6. LIN-11 protein expression and localization are regulated by SUMO modification. (A-F) Expression of the
lin-11::GFP reporter in the pi cells of wild-type and
smo-1(
ok359) animals. Nomarski photomicrographs (A,C,E) and corresponding fluorescence images (B,D,F), lateral views. (A,B) A wild-type mid-L4 stage vulva. GFP expression is shown in the pi cells (asterisk) and secondary-fate vulval cells (arrow). The six pi cells shown are arranged in two lines (outline). (C,D) A
smo-1(
ok359) animal at mid-L4 stage. (C) The unfused anchor cell is indicated by an arrowhead. (D) GFP expression is weaker and nuclear labeling decreased relative to the cytoplasmic labeling. The pi cells are clustered (outline). (E,F) A
smo-1(
ok359) animal at late L4 stage. Weak expression is detected only in one uterine pi cell (outline). (G) Immunofluorescence staining with MH27 and the anti-myc antibodies of a transgenic
smo-1(
ok359) animal expressing the
plin-11::MYC::SUMO::LIN-11 fusion protein. The upper and lower panels show the two sides of the uterus. Note six pi cells in each side of the uterus (star). LIN-11 is expressed in discrete nuclear dots (arrowhead). Arrow indicates one of the vulval cells. Bar, 10 μm.